产品英文名称：Insulin Ultrasensitive ELISA Kit产品中文名称：人胰岛素超灵敏ELISA检测试剂盒货号：80-INSHUU-E01厂家：美国ALPCO公司品牌：Alpco规格：96T/盒产品应用：人胰岛素ELISA检测保存温度： 2-8℃货期：2～5天详情请。 产品英文名称：Insulin Ultrasensitive ELISA Kit产品中文名称：人胰岛素超灵敏ELISA检测试剂盒人胰岛素介绍：胰岛素是由胰岛β细胞受内源性或外源性物质如核糖、乳糖、葡萄糖、胰 高血糖素、精氨酸等的刺激由胰腺朗格汉斯小岛所分泌的一种蛋白质激素，由A、B链组成，共含51个氨基酸残基。胰岛素是机体内*降低血糖的激素，同时促进糖原、脂肪、蛋白质合成。外源性胰岛素主要用来进行糖尿病治疗，糖尿病患者早期使用胰岛素和超强抗氧化剂如（口服虾青素、注射用硫辛酸等）有望出现较长时间的蜜月期，胰岛素注射不会有成瘾和依赖性。§ 检测原理：ALPCO人胰岛素超灵敏ELISA是一个夹层式免疫检测试剂盒。96孔微孔板涂有一层胰岛素特异性单克隆抗体。将标准物，对照品和样品一并添加到微型板小孔中与检测抗体混合。然后将微孔板放在摇床上温育1小时，700－900转/分钟。*次温育结束，用清洗缓冲液清洗小孔并烘干。添加TMB作用底物，放在微孔板摇床上第二次温育15分钟，700－900转/分钟。第二次温育结束，添加反应终止液，调节分光光度计到450nm测定OD值。由于发光强度与样品中胰岛素含量成比例，因此可以根据OD值确定胰岛素含量。§ 检测过程：各添加25 μl标准物，对照物和样品→加100µl检测抗体→微孔板摇床温育1小时，700－900rpm→清洗缓冲液清洗6次→加100µl TMB作用物→微孔板摇床温育15分钟，700－900rpm→添加100µl反应终止液→分光光度计450nm读数。Ultrasensitive Insulin ELISA英文简述：INTENDED USEThe ALPCO Ultrasensitive Insulin ELISA is designed for the quantitative determination of insulinin human serum and plasma.PRINCIPLE OF THE ASSAYThe ALPCO Ultrasensitive Insulin ELISA is a sandwich type immunoassay. The 96-well microplate is coated with a monoclonal antibody specific for insulin. The standards, samples, and the control are added to the microplate wells with the Detection Antibody. The microplate is then incubated at room temperature on a microplate shaker at 700-900 rpm. After the first incubation is complete, the wells are washed with Wash Buffer and blotted dry. TMB Substrate is added, and the microplate is incubated a second time at room temperature on a microplate shaker at 700-900 rpm. Once the second incubation is complete, Stop Solution is added, and the optical density （OD） is measured by a spectrophotometer at 450 nm. The intensity of the color generated is directly proportional to the amount of insulin in the sample.CALCULATION OF RESULTSConstruct a standard curve from the standards. The Zero Standard should be used as a blank with its average value subtracted from each well. It is recommended to use a software program to calculate the standard curve and to determine the concentration of the samples.The ALPCO Ultrasensitive Insulin ELISA is a ligand binding assay, with responses exhibiting a sigmoidal relationship to the analyte concentration. Currently accepted reference models for such curves use a 4 or 5 parameter logistic （pl） fit, as these models optimize the accuracy and precision across a greater range. Although cubic spline and other models are acceptable methods, they generally show less intra-assay accuracy and precision at the low and high ends of the range.相关产品：产品名称货号样本容量规格Insulin ELISA80-INSHU-E0125µl96T/盒Insulin Ultrasensitive ELISA80-INSHUU-E0125µl96T/盒Insulin （Synthetic） ELISA80-SINHU-E0125µl96T/盒Insulin （Mouse） ELISA80-INSMS-E0110µl96T/盒Insulin （Mouse） High Range ELISA80-INSMSH-E015µl96T/盒Insulin （Mouse） Ultrasensitive ELISA（5µl）80-INSMSU-E015µl96T/盒Insulin （Mouse） Ultrasensitive ELISA（25µl）80-INSMSU-E0125µl96T/盒Insulin （Rat） ELISA80-INSRT-E0110µl96T/盒Insulin （Rat） High Range ELISA80-INSRTH-E015µl96T/盒Insulin （Rat） Ultrasensitive ELISA（5µl）80-INSRTU-E015µl96T/盒Insulin （Rat） Ultrasensitive ELISA（25µl）80-INSRTU-E0125µl96T/盒Insulin （Bovine） ELISA80-INSBO-E0125µl96T/盒Insulin （Ovine） ELISA80-INSOV-E0125µl96T/盒Insulin （Porcine/Canine） ELISA80-INSPO-E0125µl96T/盒C-Peptide ELISA80-CPTHU-E01.125µl96T/盒C-Peptide （Mouse） ELISA80-CPTMS-E0110µl96T/盒C-Peptide （Rat） ELISA80-CPTRT-E0110µl96T/盒Proinsulin （Mouse） ELISA80-PINMS-E0110µl96T/盒Proinsulin ELISA80-PINHU-E0150µl96T/盒Proinsulin （Rat） ELISA80-PINRT-E0110µl96T/盒重庆市华雅干细胞技术有限公司